Yeast Plasma Membrane Vesicles Suitable for Transport
نویسندگان
چکیده
منابع مشابه
Water transport across yeast vacuolar and plasma membrane-targeted secretory vesicles occurs by passive diffusion.
To determine whether solute transport across yeast membranes was facilitated, we measured the water and solute permeations of vacuole-derived and late secretory vesicles in Saccharomyces cerevisiae; all permeations were consistent with passive diffusive flow. We also overexpressed Fps1p, the putative glycerol facilitator in S. cerevisiae, in secretory vesicles but observed no effect on water, g...
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Galactose transport was studied in membrane vesicles, prepared by fusion of plasma membranes from the yeast Kluyveromyces marxianus with proteoliposomes containing beef heart cytochrome c oxidase as a proton-motive force-generating system. Sugar transport studies performed under nonenergized conditions revealed that, even at high protein to phospholipid ratios, not all vesicles contained a D-ga...
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Transport of glucose and maltose was studied in plasma-membrane vesicles from Candida utilis. The yeast was grown on a mixture of glucose and maltose in aerobic carbon-limited continuous cultures which enabled transport to be studied for both sugars with the same vesicles. Vesicles were prepared by fusion of isolated plasma membranes with proteoliposomes containing bovine heart cytochrome c oxi...
متن کاملSecretory vesicles externalize the major plasma membrane ATPase in yeast
Yeast cell surface growth is accomplished by constitutive secretion and plasma membrane assembly, culminating in the fusion of vesicles with the bud membrane. Coordination of secretion and membrane assembly has been investigated by examining the biogenesis of plasma membrane ATPase (PM ATPase) in secretion-defective (sec) strains of Saccharomyces cerevisiae. PM ATPase is synthesized as a approx...
متن کاملGlucose transport in plasma membrane vesicles from rat adipose tissue.
Plasma membranes of a high degree of purity have been prepared from isolated adipose cells of rat epididymal fat pads. r+H]Glucose uptake by the membranes has been measured with a Millipore filtration technique, with L-[I%]glucose serving as a marker for nonspecific uptake or trapping of the sugars. The plasma membrane preparation shows glucose transport as evidenced by the following: (a) n-glu...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1975
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.124.3.1594-1597.1975